memerald tagged human rab11a Search Results


memerald tagged human rab11a  (Addgene inc)
93
Addgene inc memerald tagged human rab11a
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Memerald Tagged Human Rab11a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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ptag rfp c h rab11a c myc  (Addgene inc)
92
Addgene inc ptag rfp c h rab11a c myc
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Ptag Rfp C H Rab11a C Myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptag rfp c h rab11a c myc - by Bioz Stars, 2026-08
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pmemerald er 3  (Addgene inc)
93
Addgene inc pmemerald er 3
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Pmemerald Er 3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmemerald er 3 - by Bioz Stars, 2026-08
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pmdlg prre  (Addgene inc)
96
Addgene inc pmdlg prre
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Pmdlg Prre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prsv rev  (Addgene inc)
96
Addgene inc prsv rev
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Prsv Rev, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
prsv rev - by Bioz Stars, 2026-08
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lentiviral packaging envelope plasmids pmd2 g  (Addgene inc)
98
Addgene inc lentiviral packaging envelope plasmids pmd2 g
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Lentiviral Packaging Envelope Plasmids Pmd2 G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
lentiviral packaging envelope plasmids pmd2 g - by Bioz Stars, 2026-08
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memerald galt  (Addgene inc)
88
Addgene inc memerald galt
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Memerald Galt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/memerald+tagged+human+rab11a/pmc08276314-160-19-36?v=Addgene+inc
Average 88 stars, based on 1 article reviews
memerald galt - by Bioz Stars, 2026-08
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mch sec61 plasmid  (Addgene inc)
92
Addgene inc mch sec61 plasmid
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Mch Sec61 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/memerald+tagged+human+rab11a/pmc08276314-160-40-55?v=Addgene+inc
Average 92 stars, based on 1 article reviews
mch sec61 plasmid - by Bioz Stars, 2026-08
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mouse anti-pericentrin  (Becton Dickinson)
90
Becton Dickinson mouse anti-pericentrin
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Mouse Anti Pericentrin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-pericentrin - by Bioz Stars, 2026-08
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work catcaccatcttcca ggagc gapdh rt pcr r  (Addgene inc)
93
Addgene inc work catcaccatcttcca ggagc gapdh rt pcr r
a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and <t>RAB11A</t> but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.
Work Catcaccatcttcca Ggagc Gapdh Rt Pcr R, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
work catcaccatcttcca ggagc gapdh rt pcr r - by Bioz Stars, 2026-08
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Image Search Results


a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and RAB11A but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.

Journal: bioRxiv

Article Title: CK2 signaling from TOLLIP-dependent perinuclear endosomes is an essential feature of KRAS mutant cancers

doi: 10.1101/2022.04.05.487175

Figure Lengend Snippet: a , Co-immunoprecipitation assay showing association of Pyo-KSR1 with endogenous TOLLIP in 293T cells. Quantitation of three experiments is shown on the right. b , Live cell imaging shows perinuclear localization of GFP-tagged TOLLIP in A549 lung ADC cells and pan-cytoplasmic distribution in a non-transformed bronchial epithelial cell line, HBEC. Relative nuclear proximity index (RNPI) measures perinuclear clustering of fluorescent signals in each cell (see Methods). Values are averages for A549 cells (n=12) and HBEC cells (n=12). c , IF staining of A549 cells demonstrates co-localization of TOLLIP with KSR1, CK2α, and RAB11A but not p-ERK. d , TOLLIP-EGFP shows partial co-localization with KSR1-mCherry and nearly complete overlap with CK2α-mCherry and RAB11A-tagRFP in A549 cells. e , Proximity ligation assays (PLA) were used to confirm the juxtaposition of TOLLIP with KSR1, CK2α, and KSR1 but not p-ERK in A549 cells. Bottom panel: PLA signal quantification, expressed as positive signals/cell. f , Fluorescently labeled KSR1, CK2α, TOLLIP and RAB11A are present on vesicles embedded within the Sec61β-GFP positive ER network in A549 cells. Scale bars, 10 μm. Statistical significance for quantitation of immunoprecipitation and RNPI was calculated using Student’s t test; * p ≤ 0.05.

Article Snippet: The lentiviral pUltra-hot vector was a gift from Malcolm Moore (Addgene plasmid #24130; http://n2t.net/addgene:24130 ; RRID: Addgene_24130). mEmerald tagged human RAB11A and RFP tagged human RAB11A were cloned into the Ultra-hot vector using Age1 and BamH1 cloning sites.

Techniques: Co-Immunoprecipitation Assay, Quantitation Assay, Live Cell Imaging, Transformation Assay, Staining, Ligation, Labeling, Immunoprecipitation

a , TOLLIP depletion in A549 cells causes pan-cytoplasmic dispersal of KSR1, CK2α and RAB11A. b , TOLLIP ablation impairs proliferation of A549 and PANC1 tumor cells. c , TOLLIP-depleted A549 cells display increased senescence (SA-βGal positivity) and apoptosis (cleaved CASPASE-3). d , TOLLIP silencing does not affect the growth rate of HBEC cells. e , f , RAB11A knockdown results in more uniform cytoplasmic distributions of TOLLIP, KSR1 and CK2α. Two-way ANOVA was used to analyze growth curves. Student’s t-test was performed for SA-βGal assay; * p ≤ 0.05.

Journal: bioRxiv

Article Title: CK2 signaling from TOLLIP-dependent perinuclear endosomes is an essential feature of KRAS mutant cancers

doi: 10.1101/2022.04.05.487175

Figure Lengend Snippet: a , TOLLIP depletion in A549 cells causes pan-cytoplasmic dispersal of KSR1, CK2α and RAB11A. b , TOLLIP ablation impairs proliferation of A549 and PANC1 tumor cells. c , TOLLIP-depleted A549 cells display increased senescence (SA-βGal positivity) and apoptosis (cleaved CASPASE-3). d , TOLLIP silencing does not affect the growth rate of HBEC cells. e , f , RAB11A knockdown results in more uniform cytoplasmic distributions of TOLLIP, KSR1 and CK2α. Two-way ANOVA was used to analyze growth curves. Student’s t-test was performed for SA-βGal assay; * p ≤ 0.05.

Article Snippet: The lentiviral pUltra-hot vector was a gift from Malcolm Moore (Addgene plasmid #24130; http://n2t.net/addgene:24130 ; RRID: Addgene_24130). mEmerald tagged human RAB11A and RFP tagged human RAB11A were cloned into the Ultra-hot vector using Age1 and BamH1 cloning sites.

Techniques: